File:13 Hegasy CRISPR pre crRNA Wiki D CCBYSA.png
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Description13 Hegasy CRISPR pre crRNA Wiki D CCBYSA.png |
Deutsch: Transkripte des CRISPR Gen-Locus und Reifung der pre-crRNA.
1. Der CRISPR Gen-Locus von S. pyogenes besteht aus dem cas Operon, einem CRISPR Array und Genen, die für transaktivierende crRNA codieren (tracrRNA). tracrRNA, Cas9 RNA und pre-crRNA werden vom Genlocus transkribiert. tracrRNA bildet eine Struktur mit 3 Loops aus, und Cas9 Protein wird synthetisiert. 2. Da pre-crRNA als durchgehender Strang synthetisiert wird, erfolgt ein Reifungsprozess. tracrRNAs paaren sich mit den Repeat-Sequenzen der pre-crRNA. Cas9 Protein formt einen Komplex mit pre-crRNA und tracrRNA. Einzelne Komplexe werden durch Spaltung mittels RNAse III freigesetzt. 3. Der synthetisierte Interferenz-Komplex besteht aus Cas9 und einem Duplex aus tracrRNA und crRNA. |
Date | |
Source | Own work |
Author | Guido4 |
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current | 14:43, 1 November 2017 | 2,074 × 1,943 (268 KB) | Guido4 (talk | contribs) | User created page with UploadWizard |
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